Chi-Squared Test

01Chi-Squared Test

Observed and expected ratios

This section covers how observed phenotypic results are compared with values expected from a genetic ratio.

Why compare observed and expected results?

Genetic crosses predict expected phenotypic ratios, but the numbers actually obtained in an investigation may differ because fertilisation involves chance.

The chi-squared test can be used to determine whether the difference between observed and expected results is statistically significant.

  • Observed (O) values are the numbers actually recorded in each category.
  • Expected (E) values are the numbers predicted from the proposed genetic ratio.

The test is used for data divided into discrete categories. It uses raw counts rather than percentages or rates, and the sample size should be sufficiently large, with more than 20 observations.

Converting an expected ratio into numbers

Suppose a dihybrid cross predicts a phenotypic ratio of 9 : 3 : 3 : 1 and produces 128 offspring.

The ratio contains 9+3+3+1=169+3+3+1=16 parts, so one part represents:

12816=8\frac{128}{16}=8
Ratio part Calculation Expected number
9 9×89\times8 72
3 3×83\times8 24
3 3×83\times8 24
1 1×81\times8 8

The expected counts are therefore 72, 24, 24 and 8. These can now be compared with the observed counts.

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