Bacterial Data

01Bacterial Data

Logarithmic scales

This section covers why logarithmic scales are useful for rapidly changing microbial populations, how orders of magnitude are represented and how logarithmic graphs are interpreted.

Why logarithmic scales are useful

Microorganisms can reproduce rapidly in culture, so their numbers may rise from relatively small values to very large values within only a few hours. A conventional linear scale can make data covering such a wide numerical range difficult to display clearly.

A logarithmic scale allows values spanning several orders of magnitude to fit onto the same graph. This makes it particularly useful for data involving large numbers of microorganisms.

Orders of magnitude

An order of magnitude is a tenfold change in quantity. On a base-10 logarithmic scale, moving through one order of magnitude means multiplying or dividing the value by 10.

Change Interpretation
10 → 100 One order of magnitude increase
100 → 1000 One order of magnitude increase
1000 → 100 One order of magnitude decrease

Labels such as 1, 10, 100 and 1000 show that successive positions represent multiplication by the same factor rather than addition of the same numerical amount.

1.01.52.02.53.03.54.04.55.05.56.06.57.07.58.08.59.09.510.0Time / hours1231020301002003001,0002,0003,00010,000Number of cells

Interpreting microbial growth data

In the yeast-culture example, the population increases rapidly over ten hours and the number of cells spans a wide range. A logarithmic presentation allows both the smaller values near the start and the much larger later values to remain visible on one graph.

For this example, the cell-number measurements at each time interval are treated logarithmically for plotting. When reading the resulting scale, focus on the multiplicative progression of the values rather than treating neighbouring labels as equal numerical increases.

1 → 10 → 100 → 1000 → 10,000    each step = ×10

Another logarithmic scale: pH

The pH scale is logarithmic. Hydrogen ion concentration varies across a very large range between pH levels, giving another biological example of quantities that are conveniently represented logarithmically.

Exam Tip: Assessment focuses on reading results that use logarithmic scales and explaining why the scale is useful. Manual conversion of values into logarithms and construction of a logarithmic graph are not required. When interpreting an axis, look for change by a constant factor, such as ×10, rather than a constant numerical difference.