Studying Gas Exchange

01Studying Gas Exchange

Dissection

This section covers how dissection can be used to examine gas exchange structures in mammals, bony fish and insects.

Equipment and safe technique

Dissection makes it possible to inspect the larger components of animal gas exchange systems. Common tools include a scalpel, dissecting scissors, forceps and dissecting pins.

  • Use suitable protection, including a lab coat, gloves and eye protection, when handling biological material.
  • Clean, sharp instruments make controlled cutting easier and help avoid unnecessary tissue damage.
  • Keep hands clear of blade edges and make scalpel cuts in a direction away from the body.
  • Specimens should be obtained responsibly and disposed of by the appropriate laboratory procedure.

Structures that can be examined

1

Mammalian lungs

  • The trachea is distinguishable because rings of C-shaped cartilage reinforce it.
  • Its cartilage is relatively tough, so scissors may be useful when opening it.
  • The bronchi can be traced into the lungs and their finer bronchioles followed through the tissue.

The alveoli themselves are difficult to resolve by eye. Instead, the many air-containing spaces give the cut lung tissue a noticeably spongy texture.

2

Bony fish gills

Lifting the operculum reveals the gills positioned at the sides of the head.

  • A gill arch forms the firm support from which a complete gill can be removed.
  • The attached gill filaments are visible without magnification as numerous fine projections.
  • The much smaller lamellae need a microscope to be examined clearly.
3

Insect tracheal system

  1. Pin a sufficiently large insect securely onto a dissecting board.
  2. Make a longitudinal opening through the abdominal exoskeleton to expose the internal structures.
  3. Introduce saline solution into the opened abdomen; the tracheae can then be recognised more readily as shiny, silver-coloured tubes.
  4. Suitable pieces of trachea can be isolated and transferred for microscopic examination.

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